Qualitative determination of IgG antibodies to Toxoplasma gondii (T. gondii) to aid in the determination of immune status of individuals including pregnant women. If IgG antibody is detected, it is recommended that IgM testing be performed if more precise knowledge of the time of infection is needed (e.g., pregnant women). A positive IgM but a negative IgG result should be viewed with suspicion; the patient should be retested two weeks after the first test. If the patient is pregnant and IgG/IgM positive, an IgG avidity test should be performed. IgG Result IgM Result Interpretation Negative Negative No serological evidence of infection with Toxoplasma. Negative Equivocal Possible early acute infection or false‐positive IgM reaction. Obtain a new specimen for IgG and IgM testing. If results for the second specimen remain the same, the patient is probably not infected with Toxoplasma. Negative Positive Possible acute infection or false‐positive IgM result. Obtain a new specimen for IgG and IgM testing. If results for the second specimen remain the same, the IgM reaction is probably a false‐positive. Equivocal Negative Indeterminate. Obtain a new specimen for testing or retest this specimen for IgG in a different assay. Equivocal Equivocal Indeterminate. Obtain a new specimen for both IgG and IgM testing. Equivocal Positive Possible acute infection with Toxoplasma. Obtain a new specimen for IgG and IgM testing. If results for the second specimen remain the same or if the IgG becomes positive, specimens should be sent to a laboratory with expertise in toxoplasmosis for further testing. Positive Negative Infected with Toxoplasma for six months or more. Positive Equivocal Infected with Toxoplasma for probably more than one year or false‐positive IgM reaction. Obtain a new specimen for IgM testing. If results with the second specimen remain the same, specimens should be sent to a laboratory with expertise in toxoplasmosis for further testing. Positive Positive Possible recent infection within the last 12 months, or false-positive IgM reaction. Specimens should be sent to a laboratory with expertise in toxoplasmosis for further testing.